Journal
/
Immunology and Infection
/
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

English

Automatically Generated

8,348 Views

•

00:10 min

•

September 11, 2019

DOI:

10.3791/58645-v

DOI:
10.3791/58645-v

•

00:10 min
September 11, 2019

•

4 Views
, , , ,
1Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center, 2Department of Pathology and Microbiology,University of Nebraska Medical Center

Summary

Automatically generated

This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.

Explore More Videos

HIVLiver PathogenesisHumanized Mouse ModelHematopoietic Stem CellsHepatocytesUmbilical Cord BloodCD34 Positive CellsCell SeparationMagnetic Activated Cell Sorting (MACS)Cell CountingCell CultureTransplantation

Read Article

Cite this Article

Dagur, R. S., Wang, W., Makarov, E., Sun, Y., Poluektova, L. Y. Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis. J. Vis. Exp. (151), e58645, doi:10.3791/58645 (2019).

Download .ris file

Copy

Share Video

.

Copy