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Arricchire ed espandere cellule T antigene-specifiche rara con nanoparticelle magnetiche
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Engineering
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JoVE Journal Engineering
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
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Arricchire ed espandere cellule T antigene-specifiche rara con nanoparticelle magnetiche

italiano

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11,276 Views

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09:28 min

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November 17, 2018

DOI:

10.3791/58640-v

DOI:
10.3791/58640-v

•

09:28 min
November 17, 2018

•

6 Views
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1Department of Biomedical Engineering, School of Medicine,Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine,Johns Hopkins University, 3Institute for Nanobiotechnology,Johns Hopkins University, 4Department of Pathology, School of Medicine,Johns Hopkins University

Summary

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Cellule T antigene-specifiche sono difficili da caratterizzare o utilizzare in terapie grazie alla loro frequenza estremamente bassa. Qui, forniamo un protocollo per sviluppare una particella magnetica che si può associare a cellule T antigene-specifiche per arricchire queste cellule e quindi di espanderli diversi moltiplicati per la caratterizzazione e la terapia.

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Cite this Article

Hickey, J. W., Schneck, J. P. Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles. J. Vis. Exp. (141), e58640, doi:10.3791/58640 (2018).

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