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Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
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Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

DOI:
10.3791/58640-v

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09:28 min

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November 17, 2018

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,
1Department of Biomedical Engineering, School of Medicine,Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine,Johns Hopkins University, 3Institute for Nanobiotechnology,Johns Hopkins University, 4Department of Pathology, School of Medicine,Johns Hopkins University

Chapitres

  • 00:04Titre
  • 01:27Artificial Antigen Presenting Cell (aAPC) Nanoparticle Formation
  • 03:18Antigen-Specific CD8+ T Cell Enrichment and Expansion
  • 06:59Results: Representative Enrichment and Expansion of Antigen-Specific CD8+ T Cells
  • 08:45Conclusion

Summary

Traduction automatique

Traduction automatique

Antigen-specific T cells are difficult to characterize or utilize in therapies due to their extremely low frequency. Herein, we provide a protocol to develop a magnetic particle which can bind to antigen-specific T cells to enrich these cells and then to expand them several hundred-fold for both characterization and therapy.

Tags

Magnetic NanoparticlesAntigen-specific T CellsEnrichmentExpansionNeoepitopesArtificial Antigen-presenting CellsImmunotherapyCellular TherapyResuspension BufferStimulatory SignalsQuenching Buffer

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