Journal
/
Developmental Biology
/
HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

DOI:
10.3791/58591-v

•

06:12 min

•

June 24, 2019

•

, , ,
1Laboratory of Cellular and Molecular Neuropathology, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies,University of Campania “Luigi Vanvitelli”, 2Department of Molecular Medicine and Biotechnologies,University of Naples “Federico II” Naples

Chapters

  • 00:04Title
  • 00:57RGWE
  • 01:25Cell Culture and Transfection
  • 02:32Tube Formation Assay
  • 04:51Results: Rutin Alone Does Not Affect HUVEC Cell-to-Cell Network Formation
  • 05:26Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we evaluate the effects of the water extract of Ruta graveolens on vessel network formation by using a tube formation assay on a gelled basement matrix.

Tags

HUVECTube Formation AssayAngiogenesisNatural ProductsRGWEMEK/ERK PathwayEndothelial CellsCell CultureTransfectionExtracellular Matrix

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Read Article