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HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis
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Biologie du développement
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Journal JoVE Biologie du développement
HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

15,632 Views

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06:12 min

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June 24, 2019

DOI:

10.3791/58591-v

DOI:
10.3791/58591-v

•

06:12 min
June 24, 2019

•

40 Views
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1Laboratory of Cellular and Molecular Neuropathology, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies,University of Campania “Luigi Vanvitelli”, 2Department of Molecular Medicine and Biotechnologies,University of Naples “Federico II” Naples

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Here, we evaluate the effects of the water extract of Ruta graveolens on vessel network formation by using a tube formation assay on a gelled basement matrix.

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HUVECTube Formation AssayAngiogenesisNatural ProductsRGWEMEK/ERK PathwayEndothelial CellsCell CultureTransfectionExtracellular Matrix

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Cite this Article

Gentile, M. T., Pastorino, O., Bifulco, M., Colucci-D'Amato, L. HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis. J. Vis. Exp. (148), e58591, doi:10.3791/58591 (2019).

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