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利用单击化学辅助 rna-路特捕获 (无花果) 策略捕获和鉴定 rna 结合蛋白

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

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利用单击化学辅助 rna-路特捕获 (无花果) 策略捕获和鉴定 rna 结合蛋白

9,146 Views

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09:36 min

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October 19, 2018

DOI:

10.3791/58580-v

DOI:

10.3791/58580-v

•

09:36 min

October 19, 2018

•

3 Views

Rongbing Huang*^1,2, Mengting Han*^1,2, Liying Meng^1,3, Xing Chen^1,2,3,4,5

¹College of Chemistry and Molecular Engineering,Peking University, ²Beijing National Laboratory for Molecular Sciences,Peking University, ³Peking-Tsinghua Center for Life Sciences,Peking University, ⁴Synthetic and Functional Biomolecules Center,Peking University, ⁵Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University

Summary

本文介绍了应用单击化学辅助 RNA-路特捕获 (无花果) 策略来识别与编码和非编码 rna 结合的蛋白质的详细协议。

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