Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Rongbing Huang; Mengting Han; Liying Meng; Xing Chen

doi:10.3791/58580

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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

9,158 Views

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09:36 min

•

October 19, 2018

DOI:

10.3791/58580-v

DOI:

10.3791/58580-v

•

09:36 min

October 19, 2018

•

3 Views

Rongbing Huang*^1,2, Mengting Han*^1,2, Liying Meng^1,3, Xing Chen^1,2,3,4,5

¹College of Chemistry and Molecular Engineering,Peking University, ²Beijing National Laboratory for Molecular Sciences,Peking University, ³Peking-Tsinghua Center for Life Sciences,Peking University, ⁴Synthetic and Functional Biomolecules Center,Peking University, ⁵Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University

概要

A detailed protocol for applying the click chemistry-assisted RNA-interactome capture (CARIC) strategy to identify proteins binding to both coding and noncoding RNAs is presented.

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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

概要

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