Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
9,158 Views
•
09:36 min
•
October 19, 2018
DOI:
1College of Chemistry and Molecular Engineering,Peking University, 2Beijing National Laboratory for Molecular Sciences,Peking University, 3Peking-Tsinghua Center for Life Sciences,Peking University, 4Synthetic and Functional Biomolecules Center,Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University
Summary
Automatically generated
A detailed protocol for applying the click chemistry-assisted RNA-interactome capture (CARIC) strategy to identify proteins binding to both coding and noncoding RNAs is presented.
Read Article
Cite this Article
Huang, R., Han, M., Meng, L., Chen, X. Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy. J. Vis. Exp. (140), e58580, doi:10.3791/58580 (2018).
Copy