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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
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Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
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09:36 min
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October 19, 2018
DOI:
1College of Chemistry and Molecular Engineering,Peking University, 2Beijing National Laboratory for Molecular Sciences,Peking University, 3Peking-Tsinghua Center for Life Sciences,Peking University, 4Synthetic and Functional Biomolecules Center,Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University
Summary
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A detailed protocol for applying the click chemistry-assisted RNA-interactome capture (CARIC) strategy to identify proteins binding to both coding and noncoding RNAs is presented.
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Huang, R., Han, M., Meng, L., Chen, X. Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy. J. Vis. Exp. (140), e58580, doi:10.3791/58580 (2018).
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