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Label-Free Imaging di singole proteine secernuto dalle cellule viventi tramite iSCAT microscopia
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November 20, 2018
DOI:
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
Summary
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Vi presentiamo un protocollo per il rilevamento ottico in tempo reale di singole proteine senza etichetta come essi sono secernuti dalle cellule viventi. Questo si basa su microscopia interferometrica scattering (iSCAT), che può essere applicata ad una varietà di diversi sistemi biologici e configurazioni.
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Cite this Article
Gemeinhardt, A., McDonald, M. P., König, K., Aigner, M., Mackensen, A., Sandoghdar, V. Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy. J. Vis. Exp. (141), e58486, doi:10.3791/58486 (2018).
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