/
/
تنقية البروتين اللاهوائية وتحليل الحركية عبر مسرى الأوكسجين لدراسة النشاط ديوكسيجيناسي دسب وتثبيط
A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,314 Views
•
08:31 min
•
October 03, 2018
DOI:
Explore More Videos
AnaerobicProtein PurificationKinetic AnalysisOxygen ElectrodeDesB DioxygenaseEnzyme ActivityInhibitionInorganic BiochemistryOxygen-sensitive MetalsProtein ExpressionCell PelletAmylose ColumnLysozymeHigh-pressure HomogenizationNitrogen GasGlove BoxAmylose Column BufferElution BufferFerrous Ammonium SulfateDithiothreitol
Read Article
Cite this Article
Uchendu, S. N., Rafalowski, A., Cohn, E. F., Davoren, L. W., Taylor, E. A. Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition. J. Vis. Exp. (140), e58307, doi:10.3791/58307 (2018).
Copy