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Immunology and Infection
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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

English

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15,284 Views

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07:10 min

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January 07, 2019

DOI:

10.3791/58255-v

DOI:
10.3791/58255-v

•

07:10 min
January 07, 2019

•

9 Views
, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Summary

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This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

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Cite this Article

Xia, X., Li, H., Satheesan, S., Zhou, J., Rossi, J. J. Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies. J. Vis. Exp. (143), e58255, doi:10.3791/58255 (2019).

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