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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

DOI:
10.3791/58255-v

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07:10 min

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January 07, 2019

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, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Chapters

  • 00:04Title
  • 00:41Intrahepatic Hematopoietic Stem Cell (HSC) Injection
  • 01:59HSC Engraftment Validation
  • 03:48HIV Infection Analysis and Viral Suppression and Rebound Validation
  • 05:20Results: Engraftment Validation and HIV Infection and Suppression Analyses
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

Tags

Keywords: Hu-NSG Mouse ModelHIV ReplicationHIV LatencyNon-invasiveHuman Stem CellsCD34+ HSCsNOD/SCID/IL2rγnull MiceCARTHIV InfectionEngraftment ValidationFlow Cytometry

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