Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse…
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Immunology and Infection
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
 

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Article DOI: 10.3791/58255-v • 07:10 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1,2, 1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

Capítulos

Resumo
Automatic Translation

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

Tags

NOD/SCID/IL2r& 947;null Mouse Model HIV Replication HIV Latency Non-invasive Technique Hu-NSG Mice Human Stem Cells HIV Infection CART CD34+HSCs Magnetic Activated Cell Sorting Neonatal Human NOD/SCID/gamma Or NSG Mice Gamma Ray Irradiation Liver Injection Retro-orbital Blood Samples
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter