Humanized NOD/SCID/IL2r&#947;<sup>null</sup> (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Xin Xia; Haitang Li; Sangeetha Satheesan; Jiehua Zhou; John J. Rossi

doi:10.3791/58255

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Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

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JoVE Journal İmmünoloji ve Enfeksiyon

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

15,284 Views

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07:10 min

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January 07, 2019

DOI:

10.3791/58255-v

DOI:

10.3791/58255-v

•

07:10 min

January 07, 2019

•

9 Views

Xin Xia¹, Haitang Li¹, Sangeetha Satheesan^1,2, Jiehua Zhou¹, John J. Rossi¹

¹Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, ²Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Özet

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

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Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies