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A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate
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Neuroscience
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JoVE Journal Neuroscience
A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

DOI:
10.3791/58160-v

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04:48 min

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July 10, 2018

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, ,
1Chemical Biology and Therapeutics,Novartis Institutes for BioMedical Research

Chapters

  • 00:04Title
  • 00:42Measurement of NMDA-receptor Activity
  • 02:56Compound Testing
  • 03:36Results: Characterizing Activity of Ligand Binding Site Antagonist
  • 04:14Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to facilitate the study of NMDA-receptors (NMDAR) at a larger scale and allow the examination of modulatory effects of small molecules and their therapeutic applications.

Tags

NMDA ReceptorCalcium-flux AssayHigh-throughputGlycineGlutamateHEK 293 CellsCalcium 6 DyeFluorescenceLigandNeurological Diseases

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