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Vurdering af Synaptic grænsefladen af primære menneskelige T celler fra perifert blod og lymfoide væv
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1Department of Microbiology and Immunology,Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology,Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet,Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center,Thomas Jefferson University
Chapters
- 00:04Title
- 00:53Glass-supported Planar Lipid Bilayer Formation and Planar Bilayer: T Cell Interaction Imaging
- 04:39Results: Representative T Cell: Lipid Bilayer Interaction Analyses
- 05:33Conclusion
Protokollen beskriver en teknik til at studere primære polyklonale humane T celler evne til at danne synaptic grænseflader ved hjælp af planar lipid dobbeltlag. Vi bruger denne teknik til at vise den differentiale synapse dannelse evne til menneskelige primære T celler, der stammer fra lymfeknuder og perifert blod.
