/
/
Evaluación de la interfaz sináptica primaria humana de células de T de sangre periférica y tejido linfoide
A subscription to JoVE is required to view this content. Sign in or start your free trial.
6,317 Views
•
06:27 min
•
July 30, 2018
DOI:
1Department of Microbiology and Immunology,Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology,Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet,Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center,Thomas Jefferson University
Summary
Automatically generated
El protocolo describe una técnica para estudiar la capacidad del primario policlonal T las células humanas para formar sinápticas interfaces con bicapas lipídicas planares. Utilizamos esta técnica para mostrar la capacidad de formación de sinapsis diferencial de las células T humanas principal derivado de los ganglios linfáticos y la sangre periférica.
Read Article
Cite this Article
Steblyanko, M., Anikeeva, N., Buggert, M., Betts, M. R., Sykulev, Y. Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue. J. Vis. Exp. (137), e58143, doi:10.3791/58143 (2018).
Copy