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Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
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Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
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06:27 min
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July 30, 2018
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1Department of Microbiology and Immunology,Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology,Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet,Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center,Thomas Jefferson University
Summary
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The protocol describes a technique to study the ability of primary polyclonal human T cells to form synaptic interfaces using planar lipid bilayers. We use this technique to show the differential synapse formation capability of human primary T cells derived from lymph nodes and peripheral blood.
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Steblyanko, M., Anikeeva, N., Buggert, M., Betts, M. R., Sykulev, Y. Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue. J. Vis. Exp. (137), e58143, doi:10.3791/58143 (2018).
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