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A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats
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Behavior
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JoVE Journal Behavior
A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats

A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats

English

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10,361 Views

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07:04 min

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August 19, 2018

DOI:

10.3791/58129-v

DOI:
10.3791/58129-v

•

07:04 min
August 19, 2018

•

9 Views
, , ,
1Maryland Psychiatric Research Center, Department of Psychiatry,University of Maryland School of Medicine, 2Department of Pharmacology,University of Maryland School of Medicine

Summary

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Alterations in the kynurenine pathway (KP) neuroactive metabolites are implicated in psychiatric illnesses. Investigating the functional outcomes of an altered kynurenine pathway metabolism in vivo in rodents may help elucidate novel therapeutic approaches. The current protocol combines biochemical and behavioral approaches to investigate the impact of an acute kynurenine challenge in rats.

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High-performance Liquid ChromatographyKynurenineKynurenic AcidNeuroscienceRatsBiochemistryCognitionSleep

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Baratta, A. M., Viechweg, S. S., Mong, J. A., Pocivavsek, A. A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats. J. Vis. Exp. (138), e58129, doi:10.3791/58129 (2018).

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