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A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats
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A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats

A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats

DOI:
10.3791/58129-v

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07:04 min

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August 19, 2018

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, , ,
1Maryland Psychiatric Research Center, Department of Psychiatry,University of Maryland School of Medicine, 2Department of Pharmacology,University of Maryland School of Medicine

Chapters

  • 00:04Title
  • 00:40Sample Preparation
  • 02:10High-performance Liquid Chromatography (HPLC) Kynurenine Measurement
  • 05:17Results: Effects of Kynurenine Elevation on Hippocampal-dependent Memory and Sleep-wake Architecture
  • 06:44Conclusion

Summary

自动翻译

自动翻译

Alterations in the kynurenine pathway (KP) neuroactive metabolites are implicated in psychiatric illnesses. Investigating the functional outcomes of an altered kynurenine pathway metabolism in vivo in rodents may help elucidate novel therapeutic approaches. The current protocol combines biochemical and behavioral approaches to investigate the impact of an acute kynurenine challenge in rats.

Tags

Keywords: High-performance Liquid ChromatographyKynurenineKynurenic Acid神经科学Rats生物化学CognitionSleep

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