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Evaluación de respuestas de células T Zika Virus-específica en Immunoprivileged órganos de ratones- / - Ifnar1 infectados
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10:01 min
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October 17, 2018
DOI:
1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences,China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science,Chinese Academy of Sciences, 5Laboratory Animal Center,Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences
Summary
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Se describe un protocolo para evaluar las respuestas de células T antígeno específicas en los órganos de immunoprivileged del Ifnar1- / - modelo murino para la infección del virus (ZIKV) Zika. Este método es fundamental para la investigación de los mecanismos celulares de la protección y la inmunopatogénesis de las vacunas ZIKV y también es valiosa para la evaluación de su eficacia.
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Cite this Article
Zhang, Y., Zhang, H., Ma, W., Liu, K., Zhao, M., Zhao, Y., Lu, X., Zhang, F., Li, X., Gao, G. F., Liu, W. J. Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice. J. Vis. Exp. (140), e58110, doi:10.3791/58110 (2018).
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