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Valutazione delle risposte a cellula T Zika Virus-specifici in organi di nuova di Ifnar1 infetti- / - Mice
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10:01 min
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October 17, 2018
DOI:
1School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences,China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science,Chinese Academy of Sciences, 5Laboratory Animal Center,Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology,Chinese Academy of Sciences
Summary
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Un protocollo per valutare le risposte delle cellule T antigene-specifiche negli organi nuova di Ifnar1- / - modello murino per l'infezione del virus (ZIKV) di Zika è descritto. Questo metodo è fondamentale per indagare i meccanismi cellulari della protezione e immunopatogenesi di vaccini ZIKV ed è inoltre utile per la loro valutazione di efficacia.
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Cite this Article
Zhang, Y., Zhang, H., Ma, W., Liu, K., Zhao, M., Zhao, Y., Lu, X., Zhang, F., Li, X., Gao, G. F., Liu, W. J. Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice. J. Vis. Exp. (140), e58110, doi:10.3791/58110 (2018).
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