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Journal
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Biology
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衣藻
中心粒单粒子重建的分离与荧光成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation and Fluorescence Imaging for Single-particle Reconstruction of
Chlamydomonas
Centrioles
Please note that all translations are automatically generated.
Click here for the English version.
衣藻
中心粒单粒子重建的分离与荧光成像
DOI:
10.3791/58109-v
•
10:38 min
•
September 21, 2018
•
Nikolai Klena*
1
,
Davide Gambarotto*
1
,
Maeva Le Guennec
,
Susanne Borgers
,
Paul Guichard
,
Virginie Hamel
1
Department of Cell Biology, Sciences III
,
University of Geneva
Chapters
00:04
Title
00:46
Purification of
C. reinhardtii
Centrioles
05:15
Concentration of Centrioles onto the Center of the Coverslips
07:06
Quantification of Isolated Centrioles on Coverslips: Centrifugation and Immunofluorescenc
08:47
Results: Isolation and Fluorescence Imaging of
Chlamydomonas
Centrioles
10:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经制定了一个战略, 以净化和图像大量的中心粒在不同的方向, 适合超分辨率显微镜和单粒子平均。
Tags
Centriole
Chlamydomonas
Single-particle Reconstruction
Fluorescence Imaging
Isolation
Protein Localization
Deflagellation
Sucrose Cushion
Centrifugation
PBS
Lysis Buffer
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