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Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
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September 21, 2018
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We have developed a strategy to purify and image a large number of centrioles in different orientations amenable for super-resolution microscopy and single-particle averaging.
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Klena, N., Gambarotto, D., Le Guennec, M., Borgers, S., Guichard, P., Hamel, V. Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles. J. Vis. Exp. (139), e58109, doi:10.3791/58109 (2018).
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