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Time-lapse Imaging of Mitosis After siRNA Transfection

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Time-lapse Imaging of Mitosis After siRNA Transfection

Time-lapse Imaging of Mitosis After siRNA Transfection

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08:21 min

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June 06, 2010

DOI:

DOI:

•

08:21 min

June 06, 2010

•

3 Views

Douglas R. Mackay¹, Katharine S. Ullman¹, Christopher K. Rodesch²

¹Department of Oncological Sciences, Huntsman Cancer Institute,University of Utah, ²Fluorescence Microscopy Core Facility,University of Utah

Summary

Here we describe a basic protocol to image and quantify the mitotic timing of live mammalian tissue culture cells after siRNA transfection.

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