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Journal
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Immunology and Infection
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体外
犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vitro
Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
体外
犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析
DOI:
10.3791/58083-v
•
09:45 min
•
August 24, 2018
•
Ronald H.L. Li
,
Fern Tablin
1
Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine
,
University of California, Davis
,
2
Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine
,
University of California, Davis
Chapters
00:04
Title
00:34
Neutrophil Isolation
03:03
Live Neutrophil Imaging and Neutrophil Extracellular Trap (NET) Detection and Quantification
07:18
Results: Representative Canine Neutrophil Imaging and NET Detection
08:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了从全血中分离犬中性粒细胞的方法, 用荧光显微镜观察活中性粒细胞的网状形成。还描述了使用免疫荧光显微镜定量分析网络形成和瓜组蛋白 H3 (citH3) 表达的协议。
Tags
In Vitro
Canine
Neutrophil
Extracellular Trap
Formation
Fluorescence Microscopy
NET
Peroxidase
Citrullinated Histone H3
Blood
Jugular Vein
Anticoagulant
Dextran
Density Gradient Centrifugation
Erythrocytes
Polymorphonuclear Cells
Cell Culture Buffer
Trypan Blue
Poly-L-lysine
Fluorescence Microscopy
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