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Neutrophil Extracellular Traps: How to Generate and Visualize Them
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February 24, 2010
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Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.
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Neutrophil Extracellular TrapsNETsLeukocytesPhagocytesAntimicrobial GranulesGranule ProteinsChromatinPathogensCorrelative MicroscopyTEMSEMImmunofluorescenceLive Cell ImagingNucleiEu- And HeterochromatinNuclear EnvelopeGranule MembranesCell Death ProgramNETosisReactive Oxygen SpeciesNADPH OxidaseAcute InflammationInnate Immune Response
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Brinkmann, V., Laube, B., Abu Abed, U., Goosmann, C., Zychlinsky, A. Neutrophil Extracellular Traps: How to Generate and Visualize Them. J. Vis. Exp. (36), e1724, doi:10.3791/1724 (2010).
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