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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离

DOI:
10.3791/58061-v

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07:21 min

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July 29, 2018

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,
1Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 2Research Division of Immunology,Cedars-Sinai Medical Center

Chapters

  • 00:04Title
  • 00:32Magnetic-activated Cell Sorting (MACS) for Progenitor Enrichment
  • 02:06Fluorescence-activated Cell Sorting (FACS) for Myeloid Progenitor Identification and Isolation
  • 06:07Results: Representative Progenitor Yields and Purities
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

我们展示了如何用磁性和荧光分选 (mac 和流动力学) 相结合的方法, 从小鼠骨髓中识别和分离出6个髓样祖细胞。本协议可用于体外培养测定 (纤维素或液体培养),体内移植实验, RNA/蛋白质分析。

Tags

Myeloid ProgenitorsBone MarrowMACSLineage-negative CellsFACSNeutrophilsMonocytesDendritic CellsCell IsolationHematologyImmunology

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