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Un orbitale scuotendo la coltura di cellule di mammifero in vasi O a forma di produrre aggregati uniformi
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
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Un orbitale scuotendo la coltura di cellule di mammifero in vasi O a forma di produrre aggregati uniformi

italiano

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8,912 Views

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05:40 min

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January 07, 2019

DOI:

10.3791/57922-v

DOI:
10.3791/57922-v

•

05:40 min
January 07, 2019

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6 Views
, , ,
1Department of Chemical System Engineering,University of Tokyo, 2Department of Biotechnology,Osaka University, 3Biotech Business Unit,Fukoku Co. Ltd, 4Institute of Industrial Science,University of Tokyo

Summary

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Qui presentiamo un protocollo per l'utilizzo di vasi a forma di O, specializzati per colture in sospensione di aggregati cellulari, con agitazione orbitale. Le cellule HEK293 coltivate in questa borsa formano più aggregati omogenei di quelle coltivate in recipienti di coltura convenzionale.

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Cite this Article

Horiguchi, I., Suzuki, I., Morimura, T., Sakai, Y. An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates. J. Vis. Exp. (143), e57922, doi:10.3791/57922 (2019).

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