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Bioengineering

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JoVE Journal Bioengineering
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
 

An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

Article DOI: 10.3791/57922-v • 05:40 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 1,4
1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo

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Here we present a protocol for using O-shaped vessels, specialized for suspension cultures of cellular aggregates, with orbital shaking. The HEK293 cells grown in this bag form more homogeneous aggregates than those grown in conventional culture vessels.

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Orbital Shaking Culture Mammalian Cells O-shaped Vessels Uniform Aggregates Biotechnology Field Tissue Engineering Regenerative Therapy Drug Screening Suspension Culture Novel Vessels Centrifugal Force Einsteins Tea Leaf Paradox Homogenous Aggregation HEK-293 Cells Trypsin-EDTA Solution Culture Medium
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