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An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
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Bioengenharia
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JoVE Journal Bioengenharia
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates

8,907 Views

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05:40 min

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January 07, 2019

DOI:

10.3791/57922-v

DOI:
10.3791/57922-v

•

05:40 min
January 07, 2019

•

6 Views
, , ,
1Department of Chemical System Engineering,University of Tokyo, 2Department of Biotechnology,Osaka University, 3Biotech Business Unit,Fukoku Co. Ltd, 4Institute of Industrial Science,University of Tokyo

Summary

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Here we present a protocol for using O-shaped vessels, specialized for suspension cultures of cellular aggregates, with orbital shaking. The HEK293 cells grown in this bag form more homogeneous aggregates than those grown in conventional culture vessels.

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Orbital Shaking CultureMammalian CellsO-shaped VesselsUniform AggregatesDrug ScreeningSuspension CultureHEK-293 CellsCell AggregationCell CultureBioreactor Design

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Horiguchi, I., Suzuki, I., Morimura, T., Sakai, Y. An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates. J. Vis. Exp. (143), e57922, doi:10.3791/57922 (2019).

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