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August 16, 2018
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本文介绍了利用硬骨鱼类鱼鳉 (Oryzias latipes) 制作可行的脑-垂体组织切片的优化协议, 然后用膜片钳技术进行垂体细胞的电生理记录, 并穿孔补丁配置。
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Fontaine, R., Hodne, K., Weltzien, F. Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish. J. Vis. Exp. (138), e57790, doi:10.3791/57790 (2018).
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