/
/
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
Capítulos
- 00:04Título
- 00:47Tissue Harvest and Section Acquisition
- 03:06Perforated Patch-clamp and Electrophysiological Recordings
- 05:39Results: Representative Pituitary Cell Current Clamp Recordings
- 06:49Conclusion
The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.
