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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
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August 16, 2018
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The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.
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Fontaine, R., Hodne, K., Weltzien, F. Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish. J. Vis. Exp. (138), e57790, doi:10.3791/57790 (2018).
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