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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
Kapitel
- 00:04Titel
- 00:47Tissue Harvest and Section Acquisition
- 03:06Perforated Patch-clamp and Electrophysiological Recordings
- 05:39Results: Representative Pituitary Cell Current Clamp Recordings
- 06:49Conclusion
The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.
