'Dergi'
/
Genetik
/
Methodology for Accurate Detection of Mitochondrial DNA Methylation
JoVE Journal
Genetik
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Genetik
Methodology for Accurate Detection of Mitochondrial DNA Methylation

Methodology for Accurate Detection of Mitochondrial DNA Methylation

DOI:
10.3791/57772-v

•

12:11 min

•

May 20, 2018

•

, ,
1The Novo Nordisk Foundation for Basic Metabolic Research, Faculty of Health and Medical Sciences,University of Copenhagen

'Bölümler'

  • 00:05'Başlık'
  • 00:35Restriction Enzyme Treatment, Bisulfite Conversion, and Design of Bisulfite Sequencing Primers
  • 01:54Bisulfite Converted PCR and Gel Extraction
  • 04:16Bisulfite Sequencing Library Preparation
  • 10:44Results: Accurate Quantification of mtDNA Methylation
  • 11:48Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Here, we present a protocol to allow accurate quantification of mitochondrial DNA (mtDNA) methylation. In this protocol, we describe an enzymatic digestion of DNA with BamHI coupled with a bioinformatic analysis pipeline which can be used to avoid overestimation of mtDNA methylation levels caused by the secondary structure of mtDNA.

'Etiketler'

Mitochondrial DNA MethylationBisulfite SequencingPCR AmplificationPrimer DesignBiSearchMitochondrial Genome RegionsPCR ConditionsGel ElectrophoresisDNA Extraction

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Methyl-binding DNA capture Sequencing for Patient Tissues

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Detection of Copy Number Alterations Using Single Cell Sequencing

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Read Article