This study presents a validated and optimized procedure for the isolation and culture of human endometrial stromal cells to conduct in vitro decidualization assay. Further, this study provides a detailed method to efficiently knockdown a specific gene using siRNAs in human endometrial stromal cells.
Michalski, S. A., Chadchan, S. B., Jungheim, E. S., Kommagani, R. Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization. J. Vis. Exp. (139), e57684, doi:10.3791/57684 (2018).