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Isolation von menschlichen endometrialen Stromazellen Zellen für In-vitro- Decidualization
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
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Isolation von menschlichen endometrialen Stromazellen Zellen für In-vitro- Decidualization

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9,819 Views

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14:55 min

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September 01, 2018

DOI:

10.3791/57684-v

DOI:
10.3791/57684-v

•

14:55 min
September 01, 2018

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8 Views
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1Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences,Washington University School of Medicine

Summary

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Diese Studie bietet eine validierte und optimierte Verfahren zur Isolierung und Kultur menschlichen endometrialen Stromazellen Zellen in Vitro Decidualization Assay durchzuführen. Darüber hinaus bietet diese Studie einer detaillierte Methode, um effizient Knockdown ein bestimmtes Gen mit SiRNAs in menschlichen endometrialen Stromazellen Zellen.

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Michalski, S. A., Chadchan, S. B., Jungheim, E. S., Kommagani, R. Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization. J. Vis. Exp. (139), e57684, doi:10.3791/57684 (2018).

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