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超低入力ヒトゲノム単一 Tardigrade の標本からのライブラリの準備

JoVE Journal
Genetics
Author Produced

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JoVE Journal Genetics

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

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超低入力ヒトゲノム単一 Tardigrade の標本からのライブラリの準備

8,440 Views

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10:28 min

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July 15, 2018

DOI:

10.3791/57615-v

DOI:

10.3791/57615-v

•

10:28 min

July 15, 2018

•

14 Views

Yuki Yoshida^1,2, Sayuri Konno^1,3, Ryousuke Nishino^1,3, Yumi Murai^1,3, Masaru Tomita^1,2,3, Kazuharu Arakawa^1,2,3

¹Institute for Advanced Biosciences,Keio University, ²Systems Biology Program, Graduate School of Media and Governance,Keio University, ³Faculty of Environment and Information Studies,Keio University

Summary

顕微鏡の有機体のゲノムのシーケンス処理中に汚染大きい問題に残る。ここでは、汚染のリスクを最小限に抑えるため全ゲノム増幅 DNA のわずか 50 pg を単一の標本からクマムシのゲノムをシーケンス処理する方法を示します。

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