Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Yuki Yoshida; Sayuri Konno; Ryousuke Nishino; Yumi Murai; Masaru Tomita; Kazuharu Arakawa

doi:10.3791/57615

Journal

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Genetics

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Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

JoVE Journal
Genetics
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JoVE Journal Genetics

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

8,440 Views

•

10:28 min

•

July 15, 2018

DOI:

10.3791/57615-v

DOI:

10.3791/57615-v

•

10:28 min

July 15, 2018

•

14 Views

Yuki Yoshida^1,2, Sayuri Konno^1,3, Ryousuke Nishino^1,3, Yumi Murai^1,3, Masaru Tomita^1,2,3, Kazuharu Arakawa^1,2,3

¹Institute for Advanced Biosciences,Keio University, ²Systems Biology Program, Graduate School of Media and Governance,Keio University, ³Faculty of Environment and Information Studies,Keio University

Summary

Contamination during the genomic sequencing of microscopic organisms remains a large problem. Here, we show a method to sequence the genome of a tardigrade from a single specimen with as little as 50 pg of genomic DNA without whole genome amplification to minimize the risk of contamination.

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Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

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