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Genetics
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慢病毒载体介导的转基因小鼠生产: 一种简单高效的创始人直接研究方法
JoVE Journal
Genetics
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JoVE Journal
Genetics
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
Please note that all translations are automatically generated.
Click here for the English version.
慢病毒载体介导的转基因小鼠生产: 一种简单高效的创始人直接研究方法
DOI:
10.3791/57609-v
•
10:21 min
•
October 07, 2018
•
Sébastien Dussaud
2
,
Corinne Pardanaud-Glavieux
,
Claire Sauty-Colace
,
Philippe Ravassard
1
UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM
,
Sorbonne Universités
,
2
UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition
,
Sorbonne Universités
Chapters
00:04
Title
00:40
Collecting Fertilized Eggs from Superovulated Mice
02:49
Preparation of Injection Pipette Containing the Lentiviral Vector, Micro-Injection
05:04
Transferring Embryos into B6CBAF1/JRj Pseudopregnant Females
08:10
Results: Various Tested Constructs
09:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 以促进转基因基因的整合和生产的创始人转基因小鼠的高效能, 通过简单的注射慢病毒载体载体在 perivitelline 空间的受精卵母细胞。
Tags
Lentiviral
Transgenic Mice
In Vivo Screening
Gain-of-function
Loss-of-function
DNA Motif Mapping
Oviduct
Fertilized Eggs
Hyaluronidase
Cumulus Cells
M16 Medium
Lentiviral Vector
Micromanipulator
Injection Pipette
Holding Pipette
M2 Medium
Paraffin Oil
Article
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