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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

9,393 Views

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10:21 min

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October 07, 2018

DOI:

10.3791/57609-v

DOI:
10.3791/57609-v

•

10:21 min
October 07, 2018

•

3 Views
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1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM,Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition,Sorbonne Universités

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Here, we present a protocol to promote transgene integration and production of founder transgenic mice with high efficacy by a simple injection of a lentiviral vector in the perivitelline space of a fertilized oocyte.

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Dussaud, S., Pardanaud-Glavieux, C., Sauty-Colace, C., Ravassard, P. Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders. J. Vis. Exp. (140), e57609, doi:10.3791/57609 (2018).

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