We describe a protocol for detection of detergent-sensitive interactions between membrane proteins using binding of the sorting receptor, sortilin, to the first luminal loop of the glucose transporter protein, GLUT4, as an example.
Zaarur, N., Pan, X., Kandror, K. V. Detection of Detergent-sensitive Interactions Between Membrane Proteins. J. Vis. Exp. (133), e57179, doi:10.3791/57179 (2018).