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人成体和胎心标本中原发性心外膜细胞的分离培养
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Developmental Biology
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JoVE Journal Developmental Biology
The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
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人成体和胎心标本中原发性心外膜细胞的分离培养

DOI:
10.3791/57370-v

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08:57 min

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April 24, 2018

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, , , ,
1Department of Molecular Cell Biology,Leiden University Medical Center

Chapters

  • 00:04Title
  • 00:51Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
  • 04:10Culture of EPDCs
  • 05:32Induction of Epithelial to Mesenchymal Transition in EPDCs
  • 06:31Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

心外膜在心脏的发育和修复中起着至关重要的作用, 它为心肌壁提供细胞和生长因子。在这里, 我们描述了一种培养人原发性心外膜细胞的方法, 能够对它们的发育和成人特征进行研究和比较。

Tags

Epicardial CellsPrimary CellsCell CultureEpithelial-mesenchymal TransitionCell IsolationTrypsin DissociationCell SuspensionCell Strainer

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