The epicardium plays a crucial role in the development and repair of the heart by providing cells and growth factors to the myocardial wall. Here, we describe a method to culture human primary epicardial cells that enables the study and comparison of their developmental and adult characteristics.
Dronkers, E., Moerkamp, A. T., van Herwaarden, T., Goumans, M., Smits, A. M. The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens. J. Vis. Exp. (134), e57370, doi:10.3791/57370 (2018).