Journal
/
Developmental Biology
/
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

English

Automatically Generated

8,046 Views

•

07:44 min

•

March 18, 2016

DOI:

10.3791/53750-v

DOI:
10.3791/53750-v

•

07:44 min
March 18, 2016

•

2 Views
, ,
1Aab Cardiovascular Research Institute,University of Rochester School of Medicine and Dentistry, 2Department of Medicine,University of Rochester School of Medicine and Dentistry, 3Department of Pharmacology and Physiology,University of Rochester School of Medicine and Dentistry

Summary

Automatically generated

Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.

Explore More Videos

Epicardial OutgrowthEpicardial-derived Cell MigrationEx Vivo AssessmentPrimary Epicardial Cell CultureEpithelial-to-mesenchymal TransitionCardiac Regenerative MedicineCollagen-coated Chamber SlidesMouse EmbryoExplant MediumMesothelial CellsMesenchymal Cell ContaminationTrizol

Read Article

Cite this Article

Trembley, M. A., Velasquez, L. S., Small, E. M. Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration. J. Vis. Exp. (109), e53750, doi:10.3791/53750 (2016).

Download .ris file

Copy

Share Video

.

Copy