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胆汁盐诱导肠道病原菌生物膜形成的鉴别和定量技术
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
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胆汁盐诱导肠道病原菌生物膜形成的鉴别和定量技术

DOI:
10.3791/57322-v

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10:05 min

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May 06, 2018

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1Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, 2Department of Pediatrics,Harvard Medical School

Chapters

  • 00:04Title
  • 01:08Solid-phase Adherence Assay
  • 03:55Extracellular Polymeric Substance Matrix Detection
  • 05:20Dispersion Assay
  • 07:07Results: Use of Solid-phase Adherence and Extracellular Polymeric Substance Matrix Formation to Detect Biofilm Formation and Dispersion
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

该协议使读者能够分析胆汁盐诱导的肠道致病菌的生物膜形成, 采用多层面的方法来通过评估附着体、胞外高分子物质基质形成来捕获细菌生物膜的动态特性,和分散。

Tags

Bile SaltsBiofilm FormationEnteric PathogensBacterial PathogenesisShigella FlexneriCrystal Violet AssayQuantification96-well PlateStatic IncubationPBS Washing

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