Journal
/
Inmunología y contagio
/
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

12,087 Views

•

10:05 min

•

May 06, 2018

DOI:

10.3791/57322-v

DOI:
10.3791/57322-v

•

10:05 min
May 06, 2018

•

6 Views
,
1Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, 2Department of Pediatrics,Harvard Medical School

Summary

Automatically generated

This protocol enables the reader to analyze bile salt-induced biofilm formation in enteric pathogens using a multifaceted approach to capture the dynamic nature of bacterial biofilms by assessing adherence, extracellular polymeric substance matrix formation, and dispersion.

Explore More Videos

Bile SaltsBiofilm FormationEnteric PathogensBacterial PathogenesisShigella FlexneriCrystal Violet AssayQuantification96-well PlateStatic IncubationPBS Washing

Read Article

Cite this Article

Nickerson, K. P., Faherty, C. S. Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification. J. Vis. Exp. (135), e57322, doi:10.3791/57322 (2018).

Download .ris file

Copy

Share Video

.

Copy