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Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

12,087 Views

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10:05 min

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May 06, 2018

DOI:

10.3791/57322-v

DOI:
10.3791/57322-v

•

10:05 min
May 06, 2018

•

6 Views
,
1Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition,Massachusetts General Hospital, 2Department of Pediatrics,Harvard Medical School

Summary

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This protocol enables the reader to analyze bile salt-induced biofilm formation in enteric pathogens using a multifaceted approach to capture the dynamic nature of bacterial biofilms by assessing adherence, extracellular polymeric substance matrix formation, and dispersion.

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Cite this Article

Nickerson, K. P., Faherty, C. S. Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification. J. Vis. Exp. (135), e57322, doi:10.3791/57322 (2018).

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