Journal
/
Immunology and Infection
/
复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Please note that all translations are automatically generated. Click here for the English version.

复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体

DOI:
10.3791/57298-v

•

11:35 min

•

May 04, 2018

•

, , , , , , ,
1Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, 2Department of Molecular Biology,Massachusetts General Hospital, 3Department of Genetics,Harvard Medical School, 4Department of Pediatrics,Harvard Medical School

Chapters

  • 00:04Title
  • 01:26Growth of PA14NR Set Master Copy on LB Agar Plates
  • 05:02Growth of the PA14NR Set Master Copy in LB Liquid Broth
  • 07:01Transfer of PA14NR Set Overnight Cultures into Destination Plates
  • 09:43Results: Protocol Generates High-quality Copies of the PA14NR Set Mutant Library
  • 10:38Conclusion

Summary

Automatic Translation

Automatic Translation

铜绿假单胞菌感染会导致易受害主机发病率显著。非冗余座子插入突变库的绿脓杆菌菌株 PA14, 指定为 PA14NR 集, 促进分析的基因功能在许多过程中。这里提出了一个协议, 以生成高质量的副本 PA14NR 集突变库。

Tags

Pseudomonas AeruginosaPA14NR SetTransposon Mutant LibraryReplicationQuality ControlContamination PreventionSterile TechniquesReplicator Pin SterilizationLB Agar PlatesFreezer Storage

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

共培养模型绿脓杆菌现场人类呼吸道细胞生长的生物膜

铜绿假单胞菌和酿酒酵母流细胞生物膜

RNA的分离绿脓杆菌殖民小鼠胃肠道

长期慢性<em>铜绿假单胞菌</em>在呼吸道感染小鼠

在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变

绿脓杆菌引起的肺损伤模型

生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

高通量并行测序在金黄叙利亚仓鼠感染期间测量钩端螺旋体螺旋体转插入突变体的适应性

在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减

Read Article